You are here: Home » Products » Clinical&Analytical Instrument » Clinical Chemistry Reagents » 2019-nCoV RT-qPCR Detection Kit
Share to:

2019-nCoV RT-qPCR Detection Kit

This product selects the ORF1ab and N gene regions of 2019-novel coronavirus, and designs two sets of specific primers and fluorescent probes that cover two sites of the gene at the same time. In order to control the entire extraction and detection process, human β-Globin gene was act as a non-competitive internal control during the extraction and detection process. The RT-PCR reaction solution is pre-mixed, which is convenient, efficient and easy to operate. It utilizes high-efficiency Taq DNA Polymerase and has strong stability. The kit could be used for auxiliary diagnosis and epidemiological surveillance of 2019-novel coronavirus infection.

  • 2019-nCoV) RT-QPCR Detection Kit



1. Internal control: Human β-Globin gene as the internal control is included into the reagent to verify the validity of the experiment.

2. High sensitivity: The lowest detection limit is 250 copies/ml.

3. High specificity: Primers and probes are designed for specific fragments of two gene regions, which confirm each other to make the results more accurate. No cross-reactivity with other pathogens with the same site of infection or similar infection characteristics.

4. Strong stability: CV of each channel is all <3%.

5. Multiple Real-time RT-PCR detection: Each channel does not interfere with each other, and the amplification curve is S-shaped.

6. Simple operation: one-step method to complete RT-PCR, The whole procedure can be detected within 80min.

7. Fast speed: The PCR amplification time is less than 80 minutes.


Product Name

2019-Novel Coronavirus (2019-nCoV) RT-QPCR Detection Kit

Detection Principle

Fluorescence PCR

Detection Target

Novel coronavirus (2019-Ncov) ORF1ab and N gene

Sample Type

Human oropharyngeal swabs, nasopharyngeal swabs and sputum

Applicable Instrument

Fluorescence quantitative PCR instrument

Storage Conditions

-20±5℃, keep away from light

Valid Period

Unopened ≥6 months; Opened≥90 days

Sample Volume


Reaction Volume


Detection Limit

250 copies/ml


CV <3%

Interpretation of Positive Results


Packing Specification

24T/box or 48T/box

1 box/carton

Packet Size


Gross Weight



BIOBASE Group  is a new high-tech enterprise focusing on development, production and management of scientific equipment, In Vitro Diagnosis (IVD) instrument and reagent.


  6th floor, No. 2 building, 9 Gangxing road, High-tech Zone, Jinan, Shandong, China


Copyrights  2020 Biobase Biodusty(Shandong), Co., Ltd.  All rights reserved