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IMPORTANT CONSIDERATIONS WHEN CHOOSING

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A SARS-COV-2 REAL-TIME RT-PCR ASSAY

Assay Specificity

Accuracy or specificity is the ability to determine a negative case (specificity). Comparisons of commercially available real-time RT-PCR SARS-CoV-2 tests have generally concluded that they have similar accuracy. Therefore, considerations such as sensitivity, availability, throughput, labor requirements, and cost need to be considered when choosing a testing work flow

Assay Sensitivity

The sensitivity of a laboratory test determines its ability to detect a positive case. To learn how lower Limits of Detection (LOD) reduces the reporting of false-negative results. The Importance of Sensitivity in SARS-CoV-2 Real-Time RT-PCR Assays

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Reagent Availability

Availability of reagents and equipment needed for SARS-CoV-2 testing has become a critical factor that needs to be considered when choosing a solution to implement in your lab. Shortages of nucleic acid isolation reagents along with RT-PCR assays and the equipment needed to automate them have been responsible for slowing down labs ability to do testing. This can be harder to determine if multiple companies supply these components. Single source workflows simplify the ordering processes and ensure you have visibility to availability all of the workflow components.

Throughput & Labor Requirements

Throughput and labor requirements are other important factors to consider. Automated solutions help laboratories handle large numbers of samples and can be scaled to keep pace with fluctuating demand without requiring increasing the number of employees. Automation also minimizes the risk of laboratory-acquired infections during high volume processing. The steps of the SARS-CoV-2 realtime RT-PCR workflows which can be automated include:

• Sample reformatting & setup for nucleic acid isolation

• Nucleic acid isolation

• Sample Tracking

Data Analysis

Complicated qPCR systems and multiplex RT-PCR assays can be information overload. When there are simple data analysis solutions available, sample to result determination can be done with almost no effort. An easy to use Excel Macro can be configured to each laboratory’s needs and makes the huge amount of data configurable to a single report.


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