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Fluorescent Quantitative PCR Detection System

Real- time PCR is used for sensitive, specific detection and quantification of nucleic acid targets. We have developed powerful assay design algorithms, optimized qPCR regent, intuitive data analysis software, and flexible instrumentation to help harness the power of qPCR across a rich and diverse set of applications. Explore our robust solutions for your qPCR-based research.

Availability:
Quantity:
  • MA-6000
  • BIOBASE

Feature:

♦ Six independent temperature control

♦ Thermal compensation technology of temperature module

♦ No edge effect of temperature and optics have

♦ Maintenance-free long - life continuous spectral excitation source

♦ Synchronous and fast acquisition of low temperature CCD 0.15s in cold state

♦ Relative quantitative high sensitivity 1.5 times effective discrimination

♦ Flexible, open and user-friendly software platform

♦ Open reagent consumable platform


Application:

♦ Basic materials research

♦ Pathogen detection

♦ Public health safety surveillance

♦ Meat products adulterated

♦ Transgenic testing

♦ Food safety inspection

♦ Drug development and rational drug use

♦ Precise tumor treatment

♦ Gene expression

♦ Genetic screening

♦ Genotyping

♦ Stem cell research


Temperature control module

On the basis of the six independent temperature control technology, using the enclosed liquid heat transfer efficiency, combined with environmental scanning monitoring regulation auxiliary infrared with heating and intelligent variable frequency system voltage, current, ensures the module heating speed, effectively prevents temperature overshoot no edge effect and the evaporation temperature, not only saves your precious time to amplify the required temperature uniformity and the repeatability of the results achieved perfection, no matter what kind of test mode, application, it can get good data uniformity and repeatability.

01

Optical module

On behalf of the world's leading optical transmission and collection technology, use optical fiber after the excitation light source for reaction system and the emission of light from the reaction system after excitation, the energy attenuation without conduction to each reaction holes and cold CCD, from physical properties to ensure consistency and authenticity of the excitation and detection, and greatly improve the detection sensitivity, can make your low effective detection and distinguish easily copy sample. The channel mismatch function is added to extend the application field of qPCR to the protein level and provide a new way for the construction of multiple systems of diagnostic reagents.

05


Channel

Excitation

wavelength (nm)

Emission

wavelength (nm)

Examples of fluorescent dyes

1 Blue

460-480

512-528

FAM/SYBR Green I/Eva Green…

2 Green

515-535

562~578

VIC/JOE/HEX/TET…

3 Yellow

560~580

612~628

ROX/Texas Red …

4 Red

610-630

662~678

Cy5…

5 Red

660-680

702~718

Cy5.5/Quasar705…

♦ Channel combination: 6-color excitation light filter and 6-color detection light filter can detect≥21 different fluorescence spectra, channels are completely open, supporting third-party reagent optimization Support FRET application.

♦ Multichannel static fluorescence function: analysis of starting template and end product.

♦ Filter can be customized according to customer need

 

Software platform

Real-time monitoring, automatic discrimination and calculation of positive and negative results, automatic establishment of standard curve, absolute/relative quantitative, multiple quantitative, fusion curve, gene mutation, quality control graphic analysis, fluorescence correction, PCR amplification efficiency, high-resolution fusion curve (optional match), etc.

02

Models:

Technical parameters:

Product Name

Fluorescence Quantitative PCR Detection System

Model

MA-6000

Sample Capacity

96x0.2ml PCR plate, 12x8-strip tubes, 96*0.2ml single tube

Reaction System

6~125μl

Dynamics Range

1-1010 copies

Channel

5

Emission Light

Full-wavelength maintenance-free halogen tungsten lamp

Detector

-20℃ CCD

Detection Path

F1

F2

F3

F4

F5

Suitable Probe/Dye

FAM/SYBR Green/Eva Green/LC Green/Fluorescein

VIC/HEX/TET/Cy3/JOE/Alex a555

ROX/Cy3.5/Texas Red

Cy5/LC Red640

Cy5.5/LC Red705

Excitation Wavelength

380~780nm

Detection Wavelength

380~780nm

Fluorescence Detection Repeatability

CV≤3%

Fluorescence Detection Precision

CV≤ 5%

Sample Detection Repeatability

CV≤3%

Fluorescence Detection Linearity

r≥0.980

Confidence

It can effectively distinguish 5000 and 1000copies with99.8% confidence degree

Module Temp. Range

4-100℃(resolution:0.1℃) 

Heating Speed

5.0℃/s(Max), 3.5℃/ s(Avg)

Cooling Speed

5.0℃/s(Max), 3.2℃/ s(Avg)

Temp. Accuracy

±0.1℃

Temp. Uniformity

≤ ±0.25℃

Number of Temp. Control Areas

6

 

Gradient Temp. Range

1-32℃

Hot-Lid Temp. Range

104℃, Automatic Hot-lid

Special Function

Absolute quantification, Relative Quantification, Genotyping, Amplification efficiency calculation, Melting curve.

Operation System

Windows 7 64-bit or higher

Power Supply

100~240V,50~60Hz

Dimension(L*W*H) mm

600*390 *320

Port Method

USB Port

Packing Size(L*W*H) Mm

850*600 *650

Gross Weight

51

 


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