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Nucleic Acid Extraction Kit (Spin Column Method)

Introduction:
In this process, under specific buffer conditions, nucleic acids (DNA/RNA) specifically bind to silica or glass fiber membranes, while impurities such as proteins and polysaccharides are washed away. The purified nucleic acids are then eluted from the membrane, allowing for the extraction of high-purity DNA/RNA from samples. The extracted purified nucleic acids can be used in various common downstream experiments, such as enzyme digestion, reverse transcription, PCR, ligation, transformation, RT-PCR, library construction, sequencing, in vitro translation, and Southern blotting.
Availability:
Quantity:
  • Z1 Z2 Z3 Z4 Z5 Z6 Z7

  • BIOBASE

Application:

It is widely used in scientific research, medical diagnosis, animal husbandry, agriculture, bio-industry and public health.


Features:

1. High nucleic acid purity: The silica membrane specifically binds to nucleic acids under high salt conditions, efficiently removing proteins, polysaccharides, lipids, and PCR inhibitors.

2. No risk of cross-contamination: All steps are completed within the centrifuge column-collection tube system; waste liquid is passed through the membrane and discarded after each step via centrifugation.

3. No special equipment required: Only a standard benchtop centrifuge is needed.


Extraction Process:

Sample treatment with lysis buffer—nucleic acid binding to membrane—washing—elution of nucleic acid from membrane.


Advantages:

1. High concentration, high purity, and strong stability of extracted nucleic acids;

2. Convenient operation, no need for phenol-chloroform extraction, safe and environmentally friendly.


Parameters:

Item No.

Model

Specification

Applicable Sample Types

Nucleic Acid Types Extracted

DR1161

Z1

50T/Kit;

200T/Kit

Blood/cell/tissue samples

Genomic DNA

Z2

Fresh or frozen plant tissues and plant cells

Z3

Various bacterial solutions

Z4

Animal tissues preserved at -80℃ or fresh, cultured cells, suspension cells

Total RNA

Z5

Plants stored at -80℃ or fresh

Z6

Culture medium containing the target plasmid

Plasmid DNA

Z7

Agarose gel containing the target nucleic acid fragment

Single-stranded, double-stranded, or circular DNA

Package Information

20 Kits/Carton (or 40 Kits/Carton)

Package Size(W*D*H)

740*420*300mm (or 580*550*580mm)

Gross Weight

10kg (or 18kg)


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